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Protein Quantification Using ImageJ: Difference between revisions
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→Determining the concentration of protein in SDS-PAGE gel bands using ImageJ
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10. Once all lanes are defined, go to '''Analyze→Gels→Plot lanes''' (or use "Ctrl+3") to generate histograms of each lane | 10. Once all lanes are defined, go to '''Analyze→Gels→Plot lanes''' (or use "Ctrl+3") to generate histograms of each lane | ||
: [[Image:imagejhistogram.jpg|200px]] | : [[Image:imagejhistogram.jpg|200px]] | ||
: - I don't know why this one seems to be upside down, but the peaks in each grid correspond to the intensity of the bands in the land. | : - I don't know why this one seems to be upside down, but the peaks in each grid correspond to the intensity of the bands in the land. | ||
: Standards lanes should only show one peak, while lane with protein you want to quantify will probably show multiple bands, as in the image below | : Standards lanes should only show one peak, while lane with protein you want to quantify will probably show multiple bands, as in the image below | ||
: [[Image:imagejhistomultibands.jpg|200px]] | : [[Image:imagejhistomultibands.jpg|200px]] | ||
