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Protein Quantification Using ImageJ: Difference between revisions
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→Determining the concentration of protein in SDS-PAGE gel bands using ImageJ
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*To determine protein concentration you will need to have a standard curve to compare your samples to | *To determine protein concentration you will need to have a standard curve to compare your samples to | ||
:- For 5GB1, BSA works great as a protein standard, and a range of 0.025 μg/μL to 5.0 μg/μL works well as a range for the standard curve | :- For 5GB1, BSA works great as a protein standard, and a range of 0.025 μg/μL to 5.0 μg/μL works well as a range for the standard curve | ||
*After running and destaining the gel, take a picture and save it as a .tif and as a .jpg (in case the | *After running and destaining the gel, take a picture and save it as a .tif and as a .jpg (in case the tif file can’t be opened—an issue I am experiencing at the other lab). | ||
:- Make sure you save your images as the same type of .tif each time! | :- Make sure you save your gel images as the same type of image (either .jpg or .tif) each time! | ||
1. Download the ImageJ software: | 1. Download the ImageJ software: | ||
