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Biomolecular Breadboards: Difference between revisions
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Papers describing the use of the TX-TL breadboard: | Papers describing the use of the TX-TL breadboard: | ||
* [http://www.cds.caltech.edu/~murray/papers/snm13-acc.html Biomolecular resource utilization in elementary cell-free gene circuits], 2013 American Control Conference (submitted). | * [http://www.cds.caltech.edu/~murray/papers/snm13-acc.html Biomolecular resource utilization in elementary cell-free gene circuits], 2013 American Control Conference (submitted). | ||
=== Artificial cell breadboard === | |||
=== DNA origami breadboard === | === DNA origami breadboard === | ||
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A second kind of spatial organization afforded by DNA orgami is that of ''global placement''. We are developing a method for placing individual DNA origami at lithographically-patterned sites on a silicon dioxide surface: sticky patches in the shape of DNA origami are created by e-beam, origami are washed over the surface, and they bind and orient on the sticky patches. Because of the regular placement of origami, we expect to eventually be able to observe up to 1600 (40 x 40) origami localized circuits in parallel to achieve good statistics. | A second kind of spatial organization afforded by DNA orgami is that of ''global placement''. We are developing a method for placing individual DNA origami at lithographically-patterned sites on a silicon dioxide surface: sticky patches in the shape of DNA origami are created by e-beam, origami are washed over the surface, and they bind and orient on the sticky patches. Because of the regular placement of origami, we expect to eventually be able to observe up to 1600 (40 x 40) origami localized circuits in parallel to achieve good statistics. | ||
=== TX-TL modeling library === | |||
Although not strictly breadboard, we are also developing a modeling library that is a companion to the breadboards above and allows simulation of circuits, including effects of resource limits. | |||
