IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects: Difference between revisions

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*Induced when T°>42°C
*Induced when T°>42°C
  <p style="text-align:right;"> [http://2011.igem.org/Team:METU-Ankara#project5 Read More] </p>
  <p style="text-align:right;"> [http://2011.igem.org/Team:METU-Ankara#project5 Read More] </p>
|-
| <center> Caltech </center>
| <center> 2011 </center>
| <center> [http://2011.igem.org/Team:Caltech Bioremediation of Endocrine Disruptors Using Genetically Modified Escherichia Coli] </center>
| Endocrine-disrupting chemicals (EDCs) are chemicals that interact with the endocrine system by binding to hormone receptors, causing problems in sexual development and reproduction of organisms
| Biosafety ideas:
*Water filtration system for containment of microbes (filters all microbes, does not retain free DNA).
*Use the ccdB gene (BBa_P1016 and BBa_P1010)on a plasmid in conjunction with an E. coli strain such as DB3.1 (BBa_V1005). The plasmid will code for the “death gene” which will kill any cell that does not code for immunity in its genome. If a native microorganism would uptake this man-made plasmid, it would die, preventing the propagation of the recombinant DNA in the environment (Featured Parts: Cell Death).
*Another similar “suicide” containment system uses streptavidin (BBa_J36841) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade.
<p style="text-align:right;"> [http://2011.igem.org/Team:Caltech/Biosafety Read More] </p>
|-
| <center> Berkeley</center>
| <center> 2010 </center>
| <center> [http://2011.igem.org/Team:Caltech Bioremediation of Endocrine Disruptors Using Genetically Modified Escherichia Coli] </center>
| Choa Choa's Delivery Service
| Clotho Framework:
Clotho implements the current biosafety standards as outlined by the NIH and the CDC. Whenever a new part is instantiated, Clotho BLAST's its sequence against a databank of known virulence factors and pathogens and returns the RG number of the highest match. This framework ensures that every part in Clotho has a RG value associated with it. In addition, when composite parts are made by joining basic parts, the composite part is also assigned a BSL number. Finally, all strains in Clotho have a risk group.
<p style="text-align:right;"> [http://2011.igem.org/Team:Caltech/Biosafety Read More] </p>
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  | <center> Caltech </center>
  | <center> Johns Hopkins </center>
  | <center> 2011 </center>
  | <center> 2011 </center>
  | <center> [http://2011.igem.org/Team:Caltech Bioremediation of Endocrine Disruptors Using Genetically Modified Escherichia Coli] </center>
  | <center> [http://2011.igem.org/Team:Johns_Hopkins VitaYeast] </center>
  | Endocrine-disrupting chemicals (EDCs) are chemicals that interact with the endocrine system by binding to hormone receptors, causing problems in sexual development and reproduction of organisms
  | They want to make bread with yeast producing Vitamin
  | Biosafety ideas:
  | their yeast strain lacks functional pathways for '''seven''' essential aa.
*Water filtration system for containment of microbes (filters all microbes, does not retain free DNA).
  <p style="text-align:right;"> [http://2011.igem.org/Team:Johns_Hopkins/Safety Read More] </p>
*Use the ccdB gene (BBa_P1016 and BBa_P1010)on a plasmid in conjunction with an E. coli strain such as DB3.1 (BBa_V1005). The plasmid will code for the “death gene” which will kill any cell that does not code for immunity in its genome. If a native microorganism would uptake this man-made plasmid, it would die, preventing the propagation of the recombinant DNA in the environment (Featured Parts: Cell Death).
*Another similar “suicide” containment system uses streptavidin (BBa_J36841) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade.
  <p style="text-align:right;"> [http://2011.igem.org/Team:Caltech/Biosafety Read More] </p>
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  | <center> Berkeley</center>
  | <center> Kyoto </center>
  | <center> 2010 </center>
  | <center> 2009 </center>
  | <center> [http://2011.igem.org/Team:Caltech Bioremediation of Endocrine Disruptors Using Genetically Modified Escherichia Coli] </center>
  | <center> [http://2009.igem.org/Team:Kyoto/GSDD p1 : Gene Switch Depending on Duplication] </center>
  | Choa Choa's Delivery Service
  | How to express a gene after a certain lifespan. The use Linear DNA, in which a repressor will be degraded after few cell division.
  | Clotho Framework:
  | "they want to control exact cell’s life time (or '''death time''') depending on the number of cell division times.
Clotho implements the current biosafety standards as outlined by the NIH and the CDC. Whenever a new part is instantiated, Clotho BLAST's its sequence against a databank of known virulence factors and pathogens and returns the RG number of the highest match. This framework ensures that every part in Clotho has a RG value associated with it. In addition, when composite parts are made by joining basic parts, the composite part is also assigned a BSL number. Finally, all strains in Clotho have a risk group.
They get rid of the exonucelase problem by inserting multiple protein binding site that will be degraded when division occurs, but not with exonucleases. The repressor gene is degraded after a certain time."
  <p style="text-align:right;"> [http://2011.igem.org/Team:Caltech/Biosafety Read More] </p>
  <p style="text-align:right;"> [http://2009.igem.org/wiki/images/5/5f/Kyoto_GEDD_2_kai.png Read More] </p>
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Jean Cury
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