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IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects: Difference between revisions
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IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects (view source)
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*Use the ccdB gene (BBa_P1016 and BBa_P1010)on a plasmid in conjunction with an E. coli strain such as DB3.1 (BBa_V1005). The plasmid will code for the “death gene” which will kill any cell that does not code for immunity in its genome. If a native microorganism would uptake this man-made plasmid, it would die, preventing the propagation of the recombinant DNA in the environment (Featured Parts: Cell Death). | *Use the ccdB gene (BBa_P1016 and BBa_P1010)on a plasmid in conjunction with an E. coli strain such as DB3.1 (BBa_V1005). The plasmid will code for the “death gene” which will kill any cell that does not code for immunity in its genome. If a native microorganism would uptake this man-made plasmid, it would die, preventing the propagation of the recombinant DNA in the environment (Featured Parts: Cell Death). | ||
*Another similar “suicide” containment system uses streptavidin (BBa_J36841) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade. | *Another similar “suicide” containment system uses streptavidin (BBa_J36841) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade. | ||
<p style="text-align:right;"> [http://2011.igem.org/Team:Caltech/Biosafety Read More] </p> | |||
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| <center> Berkeley</center> | |||
| <center> 2010 </center> | |||
| <center> [http://2011.igem.org/Team:Caltech Bioremediation of Endocrine Disruptors Using Genetically Modified Escherichia Coli] </center> | |||
| Choa Choa's Delivery Service | |||
| Clotho Framework: | |||
Clotho implements the current biosafety standards as outlined by the NIH and the CDC. Whenever a new part is instantiated, Clotho BLAST's its sequence against a databank of known virulence factors and pathogens and returns the RG number of the highest match. This framework ensures that every part in Clotho has a RG value associated with it. In addition, when composite parts are made by joining basic parts, the composite part is also assigned a BSL number. Finally, all strains in Clotho have a risk group. | |||
<p style="text-align:right;"> [http://2011.igem.org/Team:Caltech/Biosafety Read More] </p> | <p style="text-align:right;"> [http://2011.igem.org/Team:Caltech/Biosafety Read More] </p> | ||
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