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SBB12Ntbk-JonathanKotker: Difference between revisions
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→Jon Kotker, 8 March 2012
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I performed this gel because I was not sure if the gel from [http://openwetware.org/wiki/SBB12Ntbk-JonathanKotker#Jon_Kotker.2C_6_March_2012 March 6] for the first colony was correctly done, and also I wanted to try it out with different restriction enzymes. My sample of the same colony (colony 1) was added to the eighth lane from the left. It still seems to have four distinct bands, and I am thus not going to use that colony. | I performed this gel because I was not sure if the gel from [http://openwetware.org/wiki/SBB12Ntbk-JonathanKotker#Jon_Kotker.2C_6_March_2012 March 6] for the first colony was correctly done, and also I wanted to try it out with different restriction enzymes. My sample of the same colony (colony 1) was added to the eighth lane from the left. It still seems to have four distinct bands, and I am thus not going to use that colony. | ||
'''Part pBca9525-sbb1204''': ''Picking Colonies'' | |||
The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Micro2 here] and replicated below, albeit slightly modified in step 3, for completeness. I performed another picking because the first sample (from colony 1) for the part did not seem to be correct, based on the two analytical gels that I ran for that sample. | |||
# For each construct you will pick and later miniprep 2 colonies: I picked the colonies marked 3 and 4 on the plate by Zachary for overnight incubation. | |||
# Add 4mL of LB media with the appropriate antibiotics to a clean test tube. | |||
# Pick a well-isolated, round, and "normal" looking colony with a pipette tip. | |||
# Drop it in the test tube. | |||
# Incubate at 37 overnight. | |||
