Template:SBB-Protocols Micro2
From OpenWetWare
Jump to navigationJump to search
Picking of colonies
- For each construct you will pick and later miniprep 2 colonies
- Add 4mL of LB media with the appropriate antibiotics to a clean test tube
- Pick a well-isolated, round, and "normal" looking colony with a toothpick
- Drop it in the test tube
- Incubate at 37 overnight
