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SBB12Ntbk-JonathanKotker: Difference between revisions
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→Jon Kotker, 8 March 2012
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Procedure: | Procedure: | ||
# Incubate at 37 degrees on the thermocycler for 30 minutes after approximately 11:30am. (I took it out at noon, but I unfortunately did not log the time I put the sample in the incubator.) | # Incubate at 37 degrees on the thermocycler for 30 minutes after approximately 11:30am. (I took it out at noon, but I unfortunately did not log the time I put the sample in the incubator.) | ||
The result is shown [http://openwetware.org/wiki/Image:2012_03_08_gel3_ssb2012spring_hi.jpg here] and replicated below for completeness: | |||
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_08_gel3_ssb2012spring_hi.jpg|500px]] | |||
I performed this gel because I was not sure if the gel from March 6 was correctly done, and also I wanted to try it out with different restriction enzymes. My sample of the same colony (colony 1) was added to the eighth lane from the left. It still seems to have four distinct bands, and I am thus not going to use that colony. | |||
