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SBB12Ntbk-JonathanKotker: Difference between revisions
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→Jon Kotker, 6 March 2012
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[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_06_gel2_ssb2012spring.jpg|500px]][[Image:2012_03_06_gel4_ssb2012spring.jpg|500px]] | [[Image:NEB_2-log_ladder.gif]][[Image:2012_03_06_gel2_ssb2012spring.jpg|500px]][[Image:2012_03_06_gel4_ssb2012spring.jpg|500px]] | ||
My two samples were added to the rightmost lane of the left gel and the sixth lane of the right gel. The first sample (rightmost lane of the left gel) has two distinct bands: the first band corresponds to an approximate length of 1.5kbp, while the second band corresponds to an approximate length of 3kbp, which approximately match the expected lengths of 1400bp and 2472bp. The second sample (sixth lane of the right gel) has two distinct bands: the first band corresponds to an approximate length of 1.5kbp, while the second band corresponds to an approximate length of 2kbp, which approximately match the expected lengths of 1400bp and 2472bp. | My two samples were added to the rightmost lane of the left gel and the sixth lane from the left of the right gel. The first sample (rightmost lane of the left gel) has two distinct bands: the first band corresponds to an approximate length of 1.5kbp, while the second band corresponds to an approximate length of 3kbp, which approximately match the expected lengths of 1400bp and 2472bp. The second sample (sixth lane of the right gel) has two distinct bands: the first band corresponds to an approximate length of 1.5kbp, while the second band corresponds to an approximate length of 2kbp, which approximately match the expected lengths of 1400bp and 2472bp. | ||
'''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (for the colonies picked on [http://openwetware.org/wiki/SBB12Ntbk-JonathanKotker#Jon_Kotker.2C_1_March_2012 March 1].) | '''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (for the colonies picked on [http://openwetware.org/wiki/SBB12Ntbk-JonathanKotker#Jon_Kotker.2C_1_March_2012 March 1].) | ||
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# Incubate at 37 degrees on the thermocycler for 30 minutes after 10:48am. (I took it out at 11:50am.) | # Incubate at 37 degrees on the thermocycler for 30 minutes after 10:48am. (I took it out at 11:50am.) | ||
The result is shown [http://openwetware.org/wiki/Image: | The result is shown [http://openwetware.org/wiki/Image:2012_03_06_gel2_ssb2012spring.jpg here] and http://openwetware.org/wiki/Image:2012_03_06_gel5_ssb2012spring.jpg here], and replicated below for completeness: | ||
[[Image:NEB_2-log_ladder.gif]][[Image: | [[Image:NEB_2-log_ladder.gif]][[Image:2012_03_06_gel5_ssb2012spring.jpg|500px]] | ||
I only used one of my two samples: it was added to the seventh lane from the left of the right gel. It has two distinct bands: the first band corresponds to an approximate length of 1.4kbp, while the second band corresponds to an approximate length of 2kbp, which approximately match the expected lengths of 1400bp and 2472bp. | |||
==[[User:Jonathan_Kotker|Jon Kotker]], 8 March 2012== | ==[[User:Jonathan_Kotker|Jon Kotker]], 8 March 2012== | ||
