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SBB12Ntbk-JonathanKotker: Difference between revisions
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→Jon Kotker, 6 March 2012
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'''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (samples labeled "sbb1233") | '''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (samples labeled "sbb1233") | ||
The results are shown [http://openwetware.org/wiki/Image:2012_03_06_gel2_ssb2012spring.jpg here] and [http://openwetware.org/wiki/Image: | The results are shown [http://openwetware.org/wiki/Image:2012_03_06_gel2_ssb2012spring.jpg here] and [http://openwetware.org/wiki/Image:2012_03_05_gel4_ssb2012spring.jpg here], and replicated below for completeness: | ||
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_06_gel2_ssb2012spring.jpg|500px]][[Image: | [[Image:NEB_2-log_ladder.gif]][[Image:2012_03_06_gel2_ssb2012spring.jpg|500px]][[Image:2012_03_05_gel4_ssb2012spring.jpg|500px]] | ||
My two samples were added to the sixth and seventh lane from the left. The first sample (sixth lane) seems to have four distinct bands: this is a sign of either contamination by the parent vector or an incomplete digestion. The bottom band could also be due to overflow from the adjacent lane. I am going to retry this sample later with different restriction enzymes. The second sample (seventh lane) has two distinct bands: the first band corresponds to an approximate length of 1.2kbp, while the second band corresponds to an approximate length of 3kbp, which approximately matches the expected lengths of 986bp and 2634bp. | My two samples were added to the sixth and seventh lane from the left. The first sample (sixth lane) seems to have four distinct bands: this is a sign of either contamination by the parent vector or an incomplete digestion. The bottom band could also be due to overflow from the adjacent lane. I am going to retry this sample later with different restriction enzymes. The second sample (seventh lane) has two distinct bands: the first band corresponds to an approximate length of 1.2kbp, while the second band corresponds to an approximate length of 3kbp, which approximately matches the expected lengths of 986bp and 2634bp. | ||
