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SBB12Ntbk-JonathanKotker: Difference between revisions
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→Jon Kotker, 6 March 2012
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'''Part pBca9525-sbb1204''': ''Analytical Digests for Mapping'' | '''Part pBca9525-sbb1204''': ''Analytical Digests for Mapping'' | ||
The result is shown [http://openwetware.org/wiki/Image: | The result is shown [http://openwetware.org/wiki/Image:2012_03_06_gel4_ssb2012spring.jpg here] and replicated below for completeness: | ||
[[Image:NEB_2-log_ladder.gif]][[Image: | [[Image:NEB_2-log_ladder.gif]][[Image:2012_03_06_gel4_ssb2012spring.jpg|500px]] | ||
My | My two samples were added to the sixth and seventh lane from the left. The first sample (sixth lane) seems to have four distinct bands: this is a sign of either contamination by the parent vector or an incomplete digestion. The bottom band could also be due to overflow from the adjacent lane. I am going to retry this sample later with different restriction enzymes. The second sample (seventh lane) has two distinct bands: the first band corresponds to an approximate length of 1.2kbp, while the second band corresponds to an approximate length of 2kbp. | ||
'''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (samples labeled "sbb1233") | '''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (samples labeled "sbb1233") | ||
