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SBB12Ntbk-JonathanKotker: Difference between revisions
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'''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (samples labeled "sbb1233") | '''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (samples labeled "sbb1233") | ||
'''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' ( | '''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (for the colonies picked on [http://openwetware.org/wiki/SBB12Ntbk-JonathanKotker#Jon_Kotker.2C_1_March_2012 March 1].) | ||
The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | ||
