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SBB12Ntbk-JonathanKotker: Difference between revisions
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==[[User:Jonathan_Kotker|Jon Kotker]], 6 March 2012== | ==[[User:Jonathan_Kotker|Jon Kotker]], 6 March 2012== | ||
'''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' | '''Part pBca9525-sbb1204''': ''Analytical Digests for Mapping'' | ||
The result is shown [http://openwetware.org/wiki/Image:2012_02_23_gel2_ssb2012spring_lo.jpg here] and replicated below for completeness: | |||
[[Image:NEB_2-log_ladder.gif]][[Image:2012_02_23_gel2_ssb2012spring_lo.jpg|500px]] | |||
My gel is the fourth lane from the left, having an approximate length between 500bp and 1000bp. | |||
'''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (samples labeled "sbb1233") | |||
'''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' (samples prepared on March 2) | |||
The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | ||
