233
edits
SBB12Ntbk-JonathanKotker: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Jon Kotker, 2 March 2012
| Line 327: | Line 327: | ||
The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | ||
Based on the construction file for this part, I chose the restriction enzymes EcoRI and XhoI, which should result in parts of length | Based on the construction file for this part, I chose the restriction enzymes EcoRI and XhoI, which should result in parts of length 2634bp and 986bp. The procedure is modified accordingly: | ||
Recipe: | Recipe: | ||
| Line 345: | Line 345: | ||
The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | ||
Based on the construction file for this part, I chose the restriction enzymes EcoRI and BamHI, which should result in parts of length | Based on the construction file for this part, I chose the restriction enzymes EcoRI and BamHI, which should result in parts of length 1400bp and 2472bp. The procedure is modified accordingly: | ||
Recipe: | Recipe: | ||
