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SBB12Ntbk-JonathanKotker: Difference between revisions
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'''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' | '''Part pBca9525-sbb1223''': ''Analytical Digests for Mapping'' | ||
The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | The procedure is described [http://openwetware.org/wiki/Template:SBB-Protocols_Enz6 here] and is used to guess if the purified product is indeed the DNA sequence we need. We pick particular restriction enzymes such that the resulting fragments are of significantly different sizes, which should thus show up as two distinct bands on the analytical gel. If the analytical gel shows bands at the approximately proper locations, then we can send the purified product for digestion. | ||
