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HEPES: Difference between revisions
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HEPES is a common buffering chemical similar to Tris in Tris-HCl buffers and phosphates in PBS. Buffers are used to keep the pH at a certain value and can "buffer" the addition of small amounts of acids/bases. | HEPES is a common buffering chemical similar to Tris in Tris-HCl buffers and phosphates in PBS. Buffers are used to keep the pH at a certain value and can "buffer" the addition of small amounts of acids/bases. | ||
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Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized. | Buffers are typically 1 M, prepared by neutralizing HEPES with sodium hydroxide. HEPES is essentially insoluble until it is neutralized. | ||
== 1M HEPES KOH buffer 7.5 == | |||
* 700 ml ultrapure water | |||
* 238.3 g HEPES | |||
* KOH (potassium hydroxide) pellets to adjust pH to 7.5 | |||
* to 1L w ultrapure water; sterilise by filtration | |||
== 1M HEPES-NaOH pH 7.5 == | |||
* 700 ml ultrapure water | |||
* 238.3 g HEPES | |||
* ~5.5 g NaOH (sodium hydroxide) pellets to adjust pH to 7.5 | |||
* to 1L w ultrapure water; sterilise by filtration | |||
== Links == | == Links == | ||
* original publications: Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292 | |||
* [http://en.wikipedia.org/wiki/Buffer_solution Wikipedia: buffer solution] | * [http://en.wikipedia.org/wiki/Buffer_solution Wikipedia: buffer solution] | ||
* [http://www.scribd.com/doc/12568914/Common-Buffers-Media-And-Stock-Solutions CP: common buffers] | * [http://www.scribd.com/doc/12568914/Common-Buffers-Media-And-Stock-Solutions CP: common buffers] | ||
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[[Category:Material]] | [[Category:Material]] | ||
[[Category:Buffers]] | |||
