HEPES: Difference between revisions

HEPES (view source)

117 bytes added , 2 November 2011

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-N-(2-hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS NUMBER: 7365-45-9
+[[Image:HEPES structure.png|right|thumb|Chemical structure of HEPES = N-(2-'''h'''ydroxy'''e'''thyl)<br>'''p'''iperazine-N’-<br>(2-'''e'''thane'''s'''ulfonic acid);<br>aka 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; CAS number: 7365-45-9]]
-A ''Good'' buffer [Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292].  Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.
+A ''Good'' buffer [Good'66 PMID 5942950, Good'74 PMID 4206745, Blanchard'84 PMID 6717292].
+Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range. HEPES is often used to maintain protein solubility in biochemical experiments.
 * pK<sub>a</sub> at 25C of 7.55 (7.31 at 37C)