804
edits
User:Howard Boland/Notebook/Art from Synthetic Biology/2011/01/27: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
User:Howard Boland/Notebook/Art from Synthetic Biology/2011/01/27 (view source)
Revision as of 05:03, 28 January 2011
, 28 January 2011→Flask Standard Media (FSM)
| Line 26: | Line 26: | ||
Stir to mix | Stir to mix | ||
== Flask Standard Media (FSM)== | ==Flask Standard Media (FSM)== | ||
The protocol for FSM was suggested in the paper (Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor) for growing nanomagnetic bacteria. | The protocol for FSM was suggested in the paper (Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor) for growing nanomagnetic bacteria. | ||
To make 1L FSM Media | To make 1L FSM Media | ||
| Line 45: | Line 45: | ||
Autoclave at 121ºC for 15 minutes | Autoclave at 121ºC for 15 minutes | ||
==Kligler Iron Agar== | |||
I am experimentally using this agar to see if it produces any growth, the bottle suggests using 500g powder per 9L. | |||
To make 200ml agar, 500g/9L = 500g/9000ml => 500g/ (9000ml/200ml) = 500g/45 = 11.1g | |||
# Weigh out 11.1g Powder | |||
# Add 200ml distilled water | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
