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User:Howard Boland/Notebook/Art from Synthetic Biology/2011/01/27: Difference between revisions
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User:Howard Boland/Notebook/Art from Synthetic Biology/2011/01/27 (view source)
Revision as of 04:22, 28 January 2011
, 28 January 2011→Biodetector
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== | ==Nanomagnetic Media= | ||
I am exploring suitable media for growing nanomagnetic bacteria. I will need to make both liquid broth and agar for plates. | |||
For the broth I used ready-made Brucella broth and I also prepared Flask Standard Media. I used Kligler Iron Agar for the plates. | |||
I also tried to streak out the bacteria on standard agar plates. | |||
== Bruscella Broth== | |||
28g/L, for 200ml this yields 28g/(200ml/1000ml) = 28g/5 = 5.2g | |||
# Weigh out 5.2g Bruscella Broth Powder | |||
# Add 200ml distilled water | |||
==Ferric Citrate== | |||
For the flask standard media, the last step requires ferric citrate. We did not have this in any of the labs so I tried to make some using ferric chloride and citric acid. | |||
A patent states that this is produced using 1:1 mole. Ferric Chloride (hexhydrate), a yellow toffee-like substance weighs 270.3g/mole whilst Citric Acid a sugary powder weighs 210.14g/mole. | |||
To make 10ml 1M ferric acid | |||
# Weigh out 2.7g Ferric Chloride | |||
# Weigh out 2.1g Citric Acid | |||
# Add 10ml distilled water | |||
Stir to mix, | |||
== Flask Standard Media (FSM)== | |||
# | |||
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