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Richard Lab:Restriction Digest: Difference between revisions
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Richard Lab:Restriction Digest (view source)
Revision as of 16:18, 12 January 2011
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==Notes== | ==Notes== | ||
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | ||
*DpnI eliminates the background from your PCR template | |||
*Antarctic Phosphatase eliminates background from the vector self ligating. | |||
*If you're not getting good digestion it might be because your enzyme is bad. Double the digestion time and see. | *If you're not getting good digestion it might be because your enzyme is bad. Double the digestion time and see. | ||
*Longer digest gives more complete digestion, especially if you have >1µg of DNA, but can sometimes give nonspecific digestion | *Longer digest gives more complete digestion, especially if you have >1µg of DNA, but can sometimes give nonspecific digestion | ||
