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Richard Lab:Restriction Digest: Difference between revisions
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Richard Lab:Restriction Digest (view source)
Revision as of 15:40, 12 January 2011
, 12 January 2011→Procedure
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##5μl of Buffer (usually NEBuffer 2) | ##5μl of Buffer (usually NEBuffer 2) | ||
##1μl of BSA | ##1μl of BSA | ||
##0.5 picomoles DNA (see [[DNA Quantification]]) | ##0.5 picomoles DNA (see [[DNA Quantification]])[[user:Michael A. Speer | Mike]] normally uses 10μL of miniprep or 5μL of purified PCR product | ||
##Water to make 48μl | ##Water to make 48μl | ||
# Vortex Enzymes and add 1μl (20 units) of each to the tube. | # Vortex Enzymes and add 1μl (20 units) of each to the tube. | ||
