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Richard Lab:Restriction Digest: Difference between revisions
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Richard Lab:Restriction Digest (view source)
Revision as of 16:21, 10 January 2011
, 10 January 2011→Overview
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This protocol is typically used to do bio-brick digests with the restriction sites consisting of the following configuration: | This protocol is typically used to do bio-brick digests with the restriction sites consisting of the following configuration: | ||
<center>'''-----EcoRI--XbaI--Part--SpeI--PstI-----'''</center> | <center>'''-----EcoRI--XbaI--Part--SpeI--PstI-----'''</center> | ||
This is what Mike calls "Standard Assembly 2.0" and is a method of "bio-bricking" two parts together. For more information on bio-bricking see this link. This method combines the ease standard assembly with the fidelity of triple-antibiotic (3A) assembly. The basic steps are as follows. Your two parts will be thusly labeled "insert" and "vector" although initially they will both be contained on separate plasmids. | |||
==Materials== | ==Materials== | ||
