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Pruszak:Research: Difference between revisions
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[[Image:PSCdiff.PNG|370px|thumb|left|'''Neural differentiation of hPSCs:'''<br> Exemplifying the feasibility of using hPSCs for developmental studies, we hypothesized that factors known to neuralize tissue in embryological development could be exploited to guide PSCs toward neural fate. Indeed, we were able to induce neural differentiation by the application of the BMP antagonist Noggin, and thereby greatly enhance neuralization (here: typical neuroepithelial rosettes) as well as differentiation toward functional neurons in a dose and time-dependent manner. This illustrated that, in principle, basic aspects of neural development do apply to hPSC ''in vitro'' systems. | [[Image:PSCdiff.PNG|370px|thumb|left|'''Neural differentiation of hPSCs:'''<br> Exemplifying the feasibility of using hPSCs for developmental studies, we hypothesized that factors known to neuralize tissue in embryological development could be exploited to guide PSCs toward neural fate. Indeed, we were able to induce neural differentiation by the application of the BMP antagonist Noggin, and thereby greatly enhance neuralization (here: typical neuroepithelial rosettes) as well as differentiation toward functional neurons in a dose and time-dependent manner. This illustrated that, in principle, basic aspects of neural development do apply to hPSC ''in vitro'' systems. | ||
<br> mod. from: Pruszak & Isacson, Adv Exp Med Biol 2009]] | <br> mod. from: Pruszak & Isacson, Adv Exp Med Biol 2009]] | ||
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'''Selected publications:'''<br> | '''Selected publications:'''<br> | ||
* J.E. Carette*, J. Pruszak*, M. Varadarajan, V.A. Blomen, S. Gokhale, F.D. Camargo, M. Wernig, R. Jaenisch, T.R. Brummelkamp: Generation of iPSCs from cultured human malignant cells. Blood 2010 115:4039-42. * equal contribution<br> | * J.E. Carette*, J. Pruszak*, M. Varadarajan, V.A. Blomen, S. Gokhale, F.D. Camargo, M. Wernig, R. Jaenisch, T.R. Brummelkamp: Generation of iPSCs from cultured human malignant cells. ''Blood'' '''2010''' 115:4039-42. * equal contribution<br> | ||
* S. Chung, A. Leung, B.S Han, M.Y. Chang, J.I. Moon, C.H. Kim, S. Hong, J.Pruszak, O. Isacson, K.S. Kim: Wnt1-lmx1a forms a novel autoregulatory loop and controls midbrain dopaminergic differentiation synergistically with the SHH-FoxA2 pathway. Cell Stem Cell 2009 5:646-58.<br> | * S. Chung, A. Leung, B.S Han, M.Y. Chang, J.I. Moon, C.H. Kim, S. Hong, J.Pruszak, O. Isacson, K.S. Kim: Wnt1-lmx1a forms a novel autoregulatory loop and controls midbrain dopaminergic differentiation synergistically with the SHH-FoxA2 pathway. ''Cell Stem Cell'' '''2009''' 5:646-58.<br> | ||
* J. Pruszak, W. Ludwig, A. Blak, K. Alavian, O. Isacson: CD15, CD24 and CD29 define a surface biomarker code for neural lineage differentiation of stem cells. Stem Cells 2009 27:2928-40.<br> | * J. Pruszak, W. Ludwig, A. Blak, K. Alavian, O. Isacson: CD15, CD24 and CD29 define a surface biomarker code for neural lineage differentiation of stem cells. ''Stem Cells'' '''2009''' 27:2928-40.<br> | ||
* H. Wakimoto, S. Kesari, C.J. Farrell, W.T. Curry WT, C. Zaupa, M. Aghi, T. Kuroda, A. Stemmer-Rachamimov, K. Shah, T.C. Liu, D.S. Jeyaretna, J. Debasitis, J. Pruszak, R.L. Martuza, S.D. Rabkin: Human glioblastoma-derived cancer stem cells: establishment of invasive glioma models and treatment with oncolytic herpes simplex virus vectors. Cancer Research 2009 69:3472-81.<br> | * H. Wakimoto, S. Kesari, C.J. Farrell, W.T. Curry WT, C. Zaupa, M. Aghi, T. Kuroda, A. Stemmer-Rachamimov, K. Shah, T.C. Liu, D.S. Jeyaretna, J. Debasitis, J. Pruszak, R.L. Martuza, S.D. Rabkin: Human glioblastoma-derived cancer stem cells: establishment of invasive glioma models and treatment with oncolytic herpes simplex virus vectors. ''Cancer Research'' '''2009''' 69:3472-81.<br> | ||
* J. Pruszak, O. Isacson: Molecular and cellular determinants for generating ES-cell derived dopamine neurons for cell therapy. | * J. Pruszak, O. Isacson: Molecular and cellular determinants for generating ES-cell derived dopamine neurons for cell therapy. ''Adv Exp Med Biol'' '''2009''' 651:112-23.<br> | ||
* M. Wernig, J.P. Zhao*, J. Pruszak*, E. Hedlund, D. Fu, F. Soldner, V. Broccoli, M. Constantine-Paton, O. Isacson, R. Jaenisch: Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease. PNAS 2008 105:5856-61. * equal contribution<br> | * M. Wernig, J.P. Zhao*, J. Pruszak*, E. Hedlund, D. Fu, F. Soldner, V. Broccoli, M. Constantine-Paton, O. Isacson, R. Jaenisch: Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease. ''PNAS'' '''2008''' 105:5856-61. * equal contribution<br> | ||
* E.M. Hedlund, J. Pruszak, T. Lardaro, W. Ludwig, A. Viñuela, K.S. Kim, O. Isacson: Embryonic stem (ES) cell-derived Pitx3-eGFP midbrain dopamine neurons survive enrichment by FACS and function in an animal model of Parkinson's Disease. Stem Cells 2008 26 | * E.M. Hedlund, J. Pruszak, T. Lardaro, W. Ludwig, A. Viñuela, K.S. Kim, O. Isacson: Embryonic stem (ES) cell-derived Pitx3-eGFP midbrain dopamine neurons survive enrichment by FACS and function in an animal model of Parkinson's Disease. ''Stem Cells'' '''2008''' 26:1526-36.<br> | ||
* J. Pruszak, K.C. Sonntag, M.H. Aung, R. Sanchez-Pernaute, O. Isacson: Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations. Stem Cells 2007 25 | * J. Pruszak, K.C. Sonntag, M.H. Aung, R. Sanchez-Pernaute, O. Isacson: Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations. ''Stem Cells'' '''2007''' 25:2257-68.<br> | ||
* K.C. Sonntag, J. Pruszak, T. Yoshizaki, J. van Arensbergen, R. Sanchez-Pernaute, O. Isacson: Enhanced yield of neuroepithelial precursors and midbrain-like dopaminergic neurons from human embryonic stem cells using the BMP antagonist noggin. Stem Cells 2007 25 | * K.C. Sonntag, J. Pruszak, T. Yoshizaki, J. van Arensbergen, R. Sanchez-Pernaute, O. Isacson: Enhanced yield of neuroepithelial precursors and midbrain-like dopaminergic neurons from human embryonic stem cells using the BMP antagonist noggin. ''Stem Cells'' '''2007''' 25:411-8.<br> | ||
* S. Chung, B.S. Shin, E. Hedlund, J.Pruszak, O. Isacson et al.: Genetic selection of sox1GFP-expressing neural precursors removes residual tumorigenic pluripotent stem cells and attenuates tumor formation after transplantation. J | * S. Chung, B.S. Shin, E. Hedlund, J.Pruszak, O. Isacson et al.: Genetic selection of sox1GFP-expressing neural precursors removes residual tumorigenic pluripotent stem cells and attenuates tumor formation after transplantation. ''J Neurochem'' '''2006''' 97:1467-80.<br> | ||
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