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'''The goal of our work is to enhance present knowledge of lineage specification and cell-cell interactions in human neural development. ''' Our current focus is the identification of cell-contact- as well as diffusible factor-mediated determinants of neural lineage specification. | '''The goal of our work is to enhance present knowledge of lineage specification and cell-cell interactions in human neural development. ''' Our current focus is the identification of cell-contact- as well as diffusible factor-mediated determinants of neural lineage specification. | ||
We utilize '''human pluripotent stem cells (embryonic stem cells and induced pluripotent stem cells)''' as the chief model system, complemented with primary neural cell cultures, tumor cell lines and transgenic mouse model systems. Important techniques include a number of cell and tissue culture-based assays, state-of-the-art molecular and cell biology, flow cytometry and FACS, histological analysis and microscopy. | We utilize '''human pluripotent stem cells (embryonic stem cells and induced pluripotent stem cells)''' as the chief model system, complemented with primary neural cell cultures, tumor cell lines and transgenic mouse model systems. Important techniques include a number of cell and tissue culture-based assays, state-of-the-art molecular and cell biology, flow cytometry and FACS, histological analysis and microscopy. | ||
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Human pluripotent stem cells (hPSCs) represent a valuable system to study cellular processes and disease mechanisms in phenotypes of biomedical interest and to derive cells and tissues for regenerative medicine and cell therapy. Neural differentiation of human stem cells represents a promising avenue to derive specific neuronal and glial phenotypes for potential '''cell replacement''' in the therapy of nervous system disease. Among others, therapies are being investigated for degenerative neurological disorders such as Parkinson’s and Huntington’s disease, for glial disorders such as multiple sclerosis or for trauma conditions such as spinal chord injury. PSCs are, in principle, unlimited in their expansion potential and represent an epigenetically “blank slate” that enables directed patterning toward all lineages. Importantly, the differentiation of human neural cell types from human pluripotent stem cells enables the study of '''neural development''' in an understudied model organism to date only remotely accessible to biological discovery: humans. | Human pluripotent stem cells (hPSCs) represent a valuable system to study cellular processes and disease mechanisms in phenotypes of biomedical interest and to derive cells and tissues for regenerative medicine and cell therapy. Neural differentiation of human stem cells represents a promising avenue to derive specific neuronal and glial phenotypes for potential '''cell replacement''' in the therapy of nervous system disease. Among others, therapies are being investigated for degenerative neurological disorders such as Parkinson’s and Huntington’s disease, for glial disorders such as multiple sclerosis or for trauma conditions such as spinal chord injury. PSCs are, in principle, unlimited in their expansion potential and represent an epigenetically “blank slate” that enables directed patterning toward all lineages. Importantly, the differentiation of human neural cell types from human pluripotent stem cells enables the study of '''neural development''' in an understudied model organism to date only remotely accessible to biological discovery: humans. | ||
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