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BISC 219/F10:Calendars/Planner: Difference between revisions
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→BISC219 F10 Weekly Lab Planner
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| '''Series3: Reverse Genetics''': Plasmid isolation from BL21 cells/quantification of DNA/ transformation of plasmid into HT115(DE3)cells | | '''Series3: Reverse Genetics''': Plasmid isolation from BL21 cells/quantification of DNA/ transformation of plasmid into HT115(DE3)cells | ||
| '''Day before lab:''' grow overnight culture of single colony from transformation | | '''Day before lab:''' grow overnight culture of single colony from transformation | ||
| '''Homework''': Write all Series3 Protocols as Materials & Methods. Assignment described at [[BISC_219/F10: Assignment_Series3_ Materials and Methods]]. Due at the beginning of Lab 9 ; Read the original 1998 paper by Fire and Mello published in ''Nature'' '''391''': 806-811 (Feb.19 1998) at [http://dx.doi.org/10.1038/35888 doi:10.1038/35888] ; read a | | '''Homework''': Write all Series3 Protocols as Materials & Methods. Assignment described at [[BISC_219/F10: Assignment_Series3_ Materials and Methods]]. Due at the beginning of Lab 9 ; Read the original 1998 paper by Fire and Mello published in ''Nature'' '''391''': 806-811 (Feb.19 1998) at [http://dx.doi.org/10.1038/35888 doi:10.1038/35888] ; Do your own library search to find and read a recent review article about RNAi in ''C. elegans''. Also read the 2002 Simmer ''et al.'' paper in ''Current Biology'' '''12''':1317-1319 explaining the ''rrf-3'' strain that we will use for our RNAi work at [http://dx.doi.org/10.1016/S0960-9822(02)01041-2 doi:10.1016/S0960-9822(02)01041-2 ]. These articles provide background information on how the mechanism of RNAi was worked out experimentally and will be helpful to you when you write the introduction section of your Series3 paper. Also read a published journal article by Green et al. (2009) Impact of Cigarette Smoke Exposure on Innate Immunity: A ''Caenorhabditis elegans'' Model. PLoS ONE 4(8): e6860, found at [http://dx.doi.org/10.1371/journal.pone.0006860 doi:10.1371/journal.pone.0006860]. This study, like yours, uses reverse genetics to investigate gene function. There are questions to guide your reading found at:[[BISC_219:Questions to Guide Your Reading 2]] . Be prepared to discuss those questions in Lab 9. | ||
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