9,292
edits
BISC 219/F10:Calendars/Planner: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→BISC219 F10 Weekly Lab Planner
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) |
||
| Line 128: | Line 128: | ||
| '''Series3: Reverse Genetics''': Plasmid isolation from BL21 cells/quantification of DNA/ transformation of plasmid into HT115(DE3)cells | | '''Series3: Reverse Genetics''': Plasmid isolation from BL21 cells/quantification of DNA/ transformation of plasmid into HT115(DE3)cells | ||
| '''Day before lab:''' grow overnight culture of single colony from transformation | | '''Day before lab:''' grow overnight culture of single colony from transformation | ||
| '''Homework''': Write all Series3 Protocols as Materials & Methods. Assignment described at [[BISC_219/F10: Assignment_Series3_ Materials and Methods]] ; Read the original 1998 paper by Fire and Mello published in ''Nature'' '''391''': 806-811 (Feb.19 1998) at [http://dx.doi.org/10.1038/35888 doi:10.1038/35888] ; read a 2006 review on RNAi by Yigit ''et.al'' found at [http://dx.doi.org/10.1016/j.cell.2006.09.033 doi:10.1016/j.cell.2006.09.033], and the associated perspective, [http://dx.doi.org/10.1016/j.cell.2006.11.004 doi:10.1016/j.cell.2006.11.004]], both published in ''Cell''. These articles provide background information on how the mechanism of RNAi was worked out experimentally. Also read a published journal article by Green et al. (2009) Impact of Cigarette Smoke Exposure on Innate Immunity: A Caenorhabditis elegans Model. PLoS ONE 4(8): e6860, found at [http://dx.doi.org/10.1371/journal.pone.0006860 doi:10.1371/journal.pone.0006860], that uses reverse genetics to figure out gene function. There are questions to guide your reading found at:[[BISC_219:Questions to Guide Your Reading 2]] . Be prepared to discuss those questions in Lab 9. | | '''Homework''': Write all Series3 Protocols as Materials & Methods. Assignment described at [[BISC_219/F10: Assignment_Series3_ Materials and Methods]]. Due at the beginning of Lab 9 ; Read the original 1998 paper by Fire and Mello published in ''Nature'' '''391''': 806-811 (Feb.19 1998) at [http://dx.doi.org/10.1038/35888 doi:10.1038/35888] ; read a 2006 review on RNAi by Yigit ''et.al'' found at [http://dx.doi.org/10.1016/j.cell.2006.09.033 doi:10.1016/j.cell.2006.09.033], and the associated perspective, [http://dx.doi.org/10.1016/j.cell.2006.11.004 doi:10.1016/j.cell.2006.11.004]], both published in ''Cell''. These articles provide background information on how the mechanism of RNAi was worked out experimentally. Also read a published journal article by Green et al. (2009) Impact of Cigarette Smoke Exposure on Innate Immunity: A Caenorhabditis elegans Model. PLoS ONE 4(8): e6860, found at [http://dx.doi.org/10.1371/journal.pone.0006860 doi:10.1371/journal.pone.0006860], that uses reverse genetics to figure out gene function. There are questions to guide your reading found at:[[BISC_219:Questions to Guide Your Reading 2]] . Be prepared to discuss those questions in Lab 9. | ||
|- | |- | ||
| Line 135: | Line 135: | ||
| '''Series3:RNAi''' : Induction of bacteria and seed plates for RNAi feeding; <BR> <font color="red">PAPER DISCUSSION??</font color="red"> | | '''Series3:RNAi''' : Induction of bacteria and seed plates for RNAi feeding; <BR> <font color="red">PAPER DISCUSSION??</font color="red"> | ||
| '''4 days later:''' Pick2 L4 hermaphrodite worms of N2 and ''rrf-3''genotype to 2 RNAi plates for each genotype and make 1 control 'mock"plate for each genotype (6plates); | | '''4 days later:''' Pick2 L4 hermaphrodite worms of N2 and ''rrf-3''genotype to 2 RNAi plates for each genotype and make 1 control 'mock"plate for each genotype (6plates); | ||
| '''Homework:'''Write a draft introduction section (including properly formatted Literature Cited page) of your next paper on our Reverse Genetics Project. Refer to [[BISC_219/F10:Resources]] Guide to Scientific Writing. | | '''Homework:'''Write a draft introduction section (including properly formatted Literature Cited page) of your next paper on our Reverse Genetics Project. Due at the beginning of Lab 10. Refer to [[BISC_219/F10:Resources]] Guide to Scientific Writing. | ||
|- | |- | ||
! 10 | ! 10 | ||
