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BISC 219/F10:Calendars/Planner: Difference between revisions
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→BISC219 F10 Weekly Lab Planner
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| '''Series3:Reverse Genetics''' Colony PCR to look for bacterial transformants | | '''Series3:Reverse Genetics''' Colony PCR to look for bacterial transformants | ||
| '''Day before lab:''' grow overnight culture of positive colony | | '''Day before lab:''' grow overnight culture of positive colony | ||
| '''Homework''': Continue working on your Series 2: Forward genetics paper | | '''Homework''': Continue working on your Series 2: Forward genetics paper due week of Nov 1 | ||
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! 8 | ! 8 | ||
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| '''Series3: Reverse Genetics''': Plasmid isolation from BL21 cells/quantification of DNA/ transformation of plasmid into HT115(DE3)cells | | '''Series3: Reverse Genetics''': Plasmid isolation from BL21 cells/quantification of DNA/ transformation of plasmid into HT115(DE3)cells | ||
| '''Day before lab:''' grow overnight culture of single colony from transformation | | '''Day before lab:''' grow overnight culture of single colony from transformation | ||
| '''Homework''': Write all Series3 Protocols as Materials & Methods. Assignment described at [[BISC_219/F10: Assignment_Series3_ Materials and Methods]] | | '''Homework''': Write all Series3 Protocols as Materials & Methods. Assignment described at [[BISC_219/F10: Assignment_Series3_ Materials and Methods]] ; Read the original 1998 paper by Fire and Mello published in ''Nature'' '''391''': 806-811 (Feb.19 1998) at [http://dx.doi.org/10.1038/35888 doi:10.1038/35888] ; read a 2006 review on RNAi by Yigit ''et.al'' found at [http://dx.doi.org/10.1016/j.cell.2006.09.033 doi:10.1016/j.cell.2006.09.033], and the associated perspective, [http://dx.doi.org/10.1016/j.cell.2006.11.004 doi:10.1016/j.cell.2006.11.004]], both published in ''Cell''. These articles provide background information on how the mechanism of RNAi was worked out experimentally. Also read a published journal article by Green et al. (2009) Impact of Cigarette Smoke Exposure on Innate Immunity: A Caenorhabditis elegans Model. PLoS ONE 4(8): e6860, found at [http://dx.doi.org/10.1371/journal.pone.0006860 doi:10.1371/journal.pone.0006860], that uses reverse genetics to figure out gene function. There are questions to guide your reading found at:[[BISC_219:Questions to Guide Your Reading 2]] . Be prepared to discuss those questions in Lab 9. | ||
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! 9 | ! 9 | ||
