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BISC 219/F10:Calendars/Planner: Difference between revisions
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→BISC219 F10 Weekly Lab Planner
Tucker Crum (talk | contribs) |
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| Oct. 5 to<br> Oct. 12 | | Oct. 5 to<br> Oct. 12 | ||
| '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (5 plates total);<BR> <BR> '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene | | '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (5 plates total);<BR> <BR> '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene | ||
| '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>;'''3 days after lab:''' '''Series2: Mapping:''' pick (2) <BR>heterozygotes from previous cross to each of 2 plates (4 plates total);<BR>'''Series2:Complementation:''' examine cross plates for Dpy males; | '''Homework:''' Explain complementation analysis in general and specifically how it will be used to confirm and expand your characterization of your dpy mutation. Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | | '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>;'''3 days after lab:''' '''Series2: Mapping:''' pick (2) <BR>heterozygotes from previous cross to each of 2 plates (4 plates total);<BR>'''Series2:Complementation:''' examine cross plates for Dpy males; | ||
| '''Homework:''' Explain complementation analysis in general and specifically how it will be used to confirm and expand your characterization of your dpy mutation. Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | |||
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! 6 | ! 6 | ||
