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BISC209: Preparing a bacterial smear slide: Difference between revisions
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BISC209: Preparing a bacterial smear slide (view source)
Revision as of 18:38, 27 December 2009
, 27 December 2009→Preparing a bacterial smear slide
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Preparing a bacterial smear slide is useful for microscopic examination of dead bacterial cells.<br> | Preparing a bacterial smear slide is useful for microscopic examination of dead bacterial cells.<br> | ||
1. Place a very small loopful of water on the slide | 1. Place a very small loopful of tap or deionized water on the slide (you can use the deionized water bottle on your bench-remove the cover and dip your loop in – sterility is not required for this step).<br> | ||
Note: If the bacterial smear is being made from a broth, simply place a loop or two of the broth culture on the slide and spread | ''Note:'' If the bacterial smear is being made from a broth, simply place a loop or two of the broth culture on the slide and spread, do not use additional water.<br> | ||
2. Flame the loop, cool and touch it to the colony or slant growth and place the now contaminated loop into the drop. Use large circular motions to spread the drop to about the size of a quarter over the slide.<br> | |||
2. Flame the loop, cool and touch it to the colony or slant growth and place the now contaminated loop into the drop. Use large circular motions to spread the drop to about the size of a quarter over the slide. | 3. Reflame the loop.<br> | ||
3. Reflame the loop. | 4. Be sure all the water on the slide has evaporated before proceeding to heat fixation. <br> | ||
5. Heat fix (kills and attaches organisms to slide) by rapidly passing the slide (smear side up) through a flame 3 times. Use a slide holder and avoid contact with hot glass. | |||
4. | |||
5. Heat fix ( | |||
