BISC209: Aseptic Transfer: Difference between revisions

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Knowing how to adjust the Bunsen Burner flame for the most effective incineration is extremely useful.  There are only two parts that you may need to adjust.  Watch the YouTube video for a visual demonstration.  Find the needle valve that controls the height of the flame on the bottom of the burner, check the integrity of the rubber tubing connecting the gas source to the gas inlet nozzle, and be sure to turn the gas on only when you are ready to light the burner and shut it off properly (at the gas source) whenever you are not using the flame.  
Knowing how to adjust the Bunsen Burner flame for the most effective incineration is extremely useful.  There are only two parts that you may need to adjust.  Watch the YouTube video for a visual demonstration.  Find the needle valve that controls the height of the flame on the bottom of the burner, check the integrity of the rubber tubing connecting the gas source to the gas inlet nozzle, and be sure to turn the gas on only when you are ready to light the burner and shut it off properly (at the gas source) whenever you are not using the flame.  


'''Inoculation of a Liquid Culture:'''   Broth to Broth, YouTube demo [http://www.youtube.com/watch?v=0odxJy0nR9s&feature=PlayList&p=4DA5158C3E7E14AC&playnext=1&playnext_from=PL&index=20]
'''Aseptic transfer''' <br>
Broth to Broth example, YouTube demo [http://www.youtube.com/watch?v=0odxJy0nR9s&feature=PlayList&p=4DA5158C3E7E14AC&playnext=1&playnext_from=PL&index=20]


1. Label the destination container for the culture.
1. Label the destination container for the culture.
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