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BISC209: Aseptic Transfer: Difference between revisions
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2. Select an isolated “pure” colony from your isolation streak plate. | 2. Select an isolated “pure” colony from your isolation streak plate. | ||
3. Following aseptic technique remove ½ or less of the colony using your sterilized, cooled loop by touching your loop to the surface growth. | |||
4. Holding your loop in the top 3 fingers of your dominant hand, remove the cover of a slant tube. Remember: Hold the cover in the bottom two fingers and palm while you twist the tube with your other hand. | |||
5. Insert the inoculated loop into the SLANT tube and starting at the lower surface of the agar slant, using a zig-zag or straight motion, drag the loop across and up the surface of the slant. | |||
6. Incubate at the appropriate temperature for the appropriate time. | 6. Incubate at the appropriate temperature for the appropriate time. | ||
7. Refrigerate the stock or use it for further inoculations always making at least one replacement slant first. | 7. Refrigerate the stock or use it for further inoculations always making at least one replacement slant first. | ||
