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BISC209: Aseptic Transfer: Difference between revisions
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→Aseptic Transfer
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[[Image:Asep1_0.75.jpg]] <br> | [[Image:Asep1_0.75.jpg]] <br> | ||
Figure 1: Proper flaming of a loop. Note how the handle is held by only the thumb and first two fingers and the loop is inserted into the hottest part of the flame. | Figure 1: Proper flaming of a loop. Note how the loop handle is held by only the thumb and first two fingers and the loop is inserted into the hottest part of the flame. | ||
3. Pick up the stock broth tube of your organism with your other hand, while still holding the sterile loop. With the hand holding the loop, use your little finger against your palm to remove the cover from the culture tube as shown in Figure 2. Do not put the cover down on your bench. If the tube was closed with a cotton or plastic plug, lightly pass the lip of the tube through the Bunsen burner to burn off any adhering dust. If the tube was closed with a plastic cap the lip of the tube should be sterile and this flaming is probably unnecessary. Now, insert the loop into the broth, and then remove it, carrying a loopful of culture. Flame the top of the culture tube, replace the tube cover, and return the tube to a rack.<br> | 3. Pick up the stock broth tube of your organism with your other hand, while still holding the sterile loop. With the hand holding the loop, use your little finger against your palm to remove the cover from the culture tube as shown in Figure 2. Do not put the cover down on your bench. If the tube was closed with a cotton or plastic plug, lightly pass the lip of the tube through the Bunsen burner to burn off any adhering dust. If the tube was closed with a plastic cap the lip of the tube should be sterile and this flaming is probably unnecessary. Now, insert the loop into the broth, and then remove it, carrying a loopful of culture. Flame the top of the culture tube, replace the tube cover, and return the tube to a rack.<br> | ||
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[[Image:Asep3.jpg]] <br> | [[Image:Asep3.jpg]] <br> | ||
Fig. A-3: The tube on the left uses a zig-zag pattern to encourage lots of growth. The one on the right is useful for multiple inoculation.<br> | |||
'''pure colony transfer to slant'''<br> | '''pure colony transfer to slant'''<br> | ||
1. Use the growth media appropriate for your organism | 1. Use the growth media appropriate for your organism | ||
