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BISC209: Aseptic Transfer: Difference between revisions
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→Aseptic Transfer
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5. Repeat steps 2, 3, and 5 for each liquid culture needed. | 5. Repeat steps 2, 3, and 5 for each liquid culture needed. | ||
'''Inoculation of a Slant''' | '''Inoculation of a Slant'''<br> | ||
Agar slants are made from the liquid media best suited to maintain your organism and a small amount of seaweed extract (Agar) that solidifies the media. Slants are useful for growing and storing pure cultures of organisms because evaporation is minimized and they don't spill. After growing for 24-72 hours (depending on your organism)at the appropriate temperature the agar slants is stored in the cold room. | Agar slants are made from the liquid media best suited to maintain your organism and a small amount of seaweed extract (Agar) that solidifies the media. Slants are useful for growing and storing pure cultures of organisms because evaporation is minimized and they don't spill. After growing for 24-72 hours (depending on your organism)at the appropriate temperature the agar slants is stored in the cold room. | ||
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Everytime a stock is used, the first inoculation is to prepare a replacement stock slant. This reduces the chance of contamination of your stock culture as well as maintaining a source of healthy cells over the long term. | Everytime a stock is used, the first inoculation is to prepare a replacement stock slant. This reduces the chance of contamination of your stock culture as well as maintaining a source of healthy cells over the long term. | ||
Use the inoculating loop to make the slant in either one of these 2 patterns. The zig-zag pattern allows for abundant growth when inoculating lots of media and the straight line slant is best for storing in the cold room. | Use the inoculating loop and aseptic transfer technique to make the slant in either one of these 2 patterns. The zig-zag pattern allows for abundant growth when inoculating lots of media and the straight line slant is best for storing in the cold room. | ||
[[Image:Asep3.jpg]] <br> | |||
