BISC209: Aseptic Transfer: Difference between revisions

2. Holding your loop like a pencil, insert the loop into the flame as illustrated in Figure 1.  The orientation of the loop wire in the flame is important for proper incineration. Keep the wire in the flame until it is red-hot.  Then move the adjacent nonwire part of the loop lightly through the flame.  The wire will now be sterile, and the nonwire part will have any dust burned off that might have fallen into the media during the transfer procedure.  Remember that the loop is now hot and sterile.  Allow the loop to cool for a few seconds in the air. <br>

Figure 1:  Proper flaming of a loop.  Note how the handle is held by only the thumb and first two fingers and the loop is inserted into the hottest part of the flame.