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BISC209: Aseptic Transfer: Difference between revisions
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Aseptic transfer technique is important to prevent contamination of the culture being maintained as well as yourself. Manipulation of the tubes, plates and transfer tools requires patience and practice and is vital to success in the microbiology laboratory. | Aseptic transfer technique is important to prevent contamination of the culture being maintained as well as yourself. Manipulation of the tubes, plates and transfer tools requires patience and practice and is vital to success in the microbiology laboratory. | ||
Proper use of a Bunsen burner: YouTube demo [http://www. | Proper use of a Bunsen burner: YouTube demo [http://www.youtube.com/watch?v=iHNmJGcd89A&feature=PlayList&p=4DA5158C3E7E14AC&index=23&playnext=4&playnext_from=PL] | ||
Knowing how to adjust the Bunsen Burner flame for the most effective incineration is extremely useful. There are only two parts that you may need to adjust. Watch the YouTube video for a visual demonstration. Find the needle valve that controls the height of the flame on the bottom of the burner, check the integrity of the rubber tubing connecting the gas source to the gas inlet nozzle, and be sure to turn the gas on only when you are ready to light the burner and shut it off properly (at the gas source) whenever you are not using the flame. | Knowing how to adjust the Bunsen Burner flame for the most effective incineration is extremely useful. There are only two parts that you may need to adjust. Watch the YouTube video for a visual demonstration. Find the needle valve that controls the height of the flame on the bottom of the burner, check the integrity of the rubber tubing connecting the gas source to the gas inlet nozzle, and be sure to turn the gas on only when you are ready to light the burner and shut it off properly (at the gas source) whenever you are not using the flame. | ||
Inoculation of a Liquid Culture: Broth to Broth, YouTube demo | Inoculation of a Liquid Culture: Broth to Broth, YouTube demo [http://www.youtube.com/watch?v=0odxJy0nR9s&feature=PlayList&p=4DA5158C3E7E14AC&playnext=1&playnext_from=PL&index=20] | ||
1. Label the destination container for the culture. | 1. Label the destination container for the culture. | ||
