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In vitro transcribed RNAs like those from T7 or similar viral promoters like T3 and SP6 are important components for many molecular biology experiments. They can be used to generate (antisense) RNA probes for blot hybridisation and nuclease protection assays that are more sensitive than randomly primed DNA probes. Modified nucleotides containing isotopes like <sup>32</sup>P or detectable epitopes like DIG can be integrated into the RNA via T7 transcription. Synthesis can be scaled up for microinjection, viral RNA infection studies, in vitro translation, and binding experiments. | In vitro transcribed RNAs like those from T7 or similar viral promoters like T3 and SP6 are important components for many molecular biology experiments. They can be used to generate (antisense) RNA probes for blot hybridisation and nuclease protection assays that are more sensitive than randomly primed DNA probes. Modified nucleotides containing isotopes like <sup>32</sup>P or detectable epitopes like DIG can be integrated into the RNA via T7 transcription. Synthesis can be scaled up for microinjection, viral RNA infection studies, in vitro translation, and binding experiments. | ||
The [[Sauer lab]] has an excellent, detailed protocol: [[Sauer:In vitro transcription with T7 RNA polymerase]]. | |||
* [[Knight:In vitro transcription]] | For a detailed description of PCR-based attachment of T7 promoters see [[Making RNA probes with T7 transcription]]. | ||
== See also == | |||
* [[Knight:In vitro transcription|Knight:In vitro transcription with E. coli RNA polymerase]] | |||
== Related OWW pages == | == Related OWW pages == |
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