RNA extraction using trizol/tri: Difference between revisions

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m
→‎Reagents: removed details and instead placed link to more extensive DEPC page
m (→‎Reagents: moved to separate protocol page to keep the page shorter)
m (→‎Reagents: removed details and instead placed link to more extensive DEPC page)
Line 18: Line 18:
* chloroform
* chloroform
* 75% EtOH in DEPC H2O
* 75% EtOH in DEPC H2O
* RNase free water (filtered or DEPC)
* RNase free water (filtered or [[DEPC]])
 
<small>
<pre>
draw water into RNase-free glass bottles
add diethylpyrocarbonate (DEPC) to 0.01% (v/v)
let stand overnight and autoclave
</pre>
</small>


== Steps ==
== Steps ==
2,879

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