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DEPC: Difference between revisions
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→DEPC-treatment of solutions
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==DEPC-treatment of solutions== | ==DEPC-treatment of solutions== | ||
*add 0.05-0.1% (v/v) DEPC to solution/water | *add 0.05-0.1% (v/v) DEPC to solution/water | ||
<small>( | <small>(More DEPC is needed to inactivate larger amounts of RNases, but larger amount of RNases take longer to remove subsequently and increase the risk of residual DEPC, health risk, and interference with downstream applications. 0.05% is recommended, for example, in ''RNA methodologies'' by Farrell, whereas 1% is probably the most repeated number.)</small> | ||
*shake for 1h to O/N on orbital shaker OR 20-30min with a magnetic stirrer | *shake for 1h to O/N on orbital shaker OR 20-30min with a magnetic stirrer | ||
*DEPC must then be completely destroyed by autoclaving (15min at 15psi on liquid cycle) | *DEPC must then be completely destroyed by autoclaving (15min at 15psi on liquid cycle) | ||
