118
edits
DNA extraction - Salting Out: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
DNA extraction - Salting Out (view source)
Revision as of 18:12, 15 December 2008
, 15 December 2008→Notes
(→Notes) |
|||
| Line 51: | Line 51: | ||
==Notes== | ==Notes== | ||
*Other Salts (NaCl, AmOAc, LiCl) may also be used. See here for an explanation of how ethanol precipitation works and a description of the roles of different salts [http://bitesizebio.com/2007/12/04/the-basics-how-ethanol-precipitation-of-dna-and-rna-works/] | *Other Salts (NaCl, AmOAc, LiCl) may also be used. See here for an explanation of how ethanol precipitation works and a description of the roles of different salts [http://bitesizebio.com/2007/12/04/the-basics-how-ethanol-precipitation-of-dna-and-rna-works/][http://labmom.com/protocol/isopropanol_precipitation] | ||
*I routinely rely on the denaturation step of Hot-start Taq in my PCR to take care killing proteinase K (unless it's just not carrying over through the precipitations, either way it hasn't been a problem for me in PCR downstream). | *I routinely rely on the denaturation step of Hot-start Taq in my PCR to take care killing proteinase K (unless it's just not carrying over through the precipitations, either way it hasn't been a problem for me in PCR downstream). | ||
*I have successfully used this protocol to prepare quality PCR template from fish, mammal and insect tissue. | *I have successfully used this protocol to prepare quality PCR template from fish, mammal and insect tissue. | ||
