118
edits
DNA extraction - Salting Out: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
DNA extraction - Salting Out (view source)
Revision as of 06:04, 26 November 2008
, 26 November 2008→Notes
(→Notes) |
(→Notes) |
||
| Line 55: | Line 55: | ||
*I have successfully used this protocol to prepare quality PCR template from fish, mammal and insect tissue. | *I have successfully used this protocol to prepare quality PCR template from fish, mammal and insect tissue. | ||
*Works well for high throughput (e.g. 96 well plate extractions). To remove ethanol following precipitation/wash steps simply invert the plate over a sink then spin gently (~100 rcf) inverted over absorbant paper towelling. | *Works well for high throughput (e.g. 96 well plate extractions). To remove ethanol following precipitation/wash steps simply invert the plate over a sink then spin gently (~100 rcf) inverted over absorbant paper towelling. | ||
*To increase the efficiency of the ethanol washes you may vortex to dislodge the pellet and re-centrifuge (5-10 minutes) | |||
==Acknowledgments== | ==Acknowledgments== | ||
