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DNA extraction - Salting Out: Difference between revisions
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→Procedure
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#Invert to mix and incubate at -20°C for ~15 minutes | #Invert to mix and incubate at -20°C for ~15 minutes | ||
#Centrifuge (preferably at 4°C) at maximum speed in a benchtop centrifuge for 20 minutes | #Centrifuge (preferably at 4°C) at maximum speed in a benchtop centrifuge for 20 minutes | ||
#Wash pellet with | #Wash pellet with 98% ethanol, and once or twice with 70%. Allow to air dry then resuspend in water or 1xTE | ||
==Critical steps== | ==Critical steps== | ||
