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DNA extraction - Salting Out: Difference between revisions
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→Procedure
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*Precipitation of Nucleic Acids | *Precipitation of Nucleic Acids | ||
#Add | #Add ~2 volumes of 98% ethanol (final 60-80%) | ||
#Invert to mix and incubate at -20°C for ~15 minutes | #Invert to mix and incubate at -20°C for ~15 minutes | ||
#Centrifuge (preferably at 4°C) at maximum speed in a benchtop centrifuge for 20 minutes | #Centrifuge (preferably at 4°C) at maximum speed in a benchtop centrifuge for 20 minutes | ||
