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DNA extraction - Salting Out: Difference between revisions
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==Notes== | ==Notes== | ||
*I routinely rely on the denaturation step of Hot-start Taq in my PCR to take care killing proteinase K | *Other Salts (NaCl, AmOAc, LiCl) may also be used. See here for an explanation of how ethanol precipitation works and a descrition of the role of varying salts [http://bitesizebio.com/2007/12/04/the-basics-how-ethanol-precipitation-of-dna-and-rna-works/] | ||
* | *I routinely rely on the denaturation step of Hot-start Taq in my PCR to take care killing proteinase K | ||
*Works well | *I have successfully used this protocol on fish, mammal and insect tissue. | ||
*Works well for high throughput (e.g. 96 well plate extractions). To remove ethanol following precipitation/wash steps simply invert the plate over a sink then spin gently (~100 rcf) inverted over absorbant paper towelling. | |||
==Acknowledgments== | ==Acknowledgments== | ||
