484
edits
TOP10 chemically competent cells: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Measurement of competence
Felix Moser (talk | contribs) |
Felix Moser (talk | contribs) |
||
| Line 78: | Line 78: | ||
** Good cells should yield around 100 - 400 colonies | ** Good cells should yield around 100 - 400 colonies | ||
** Transformation efficiency is (dilution factor=15) x colony count x 10<sup>5</sup> cfu/µgDNA | ** Transformation efficiency is (dilution factor=15) x colony count x 10<sup>5</sup> cfu/µgDNA | ||
**We expect that the transformation efficiency should be between 5x10<sup>8</sup> and 5x10<sup>9</sup> | **We expect that the transformation efficiency should be between 5x10<sup>8</sup> and 5x10<sup>9</sup> cfu/µgDNA | ||
==5x Ligation Adjustment Buffer== | ==5x Ligation Adjustment Buffer== | ||
