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SOC Medium. Used in Bacterial Transformation.
- 20 mM glucose
- Follow directions to make 1 liter of SOB media
- After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
- 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
- aliquote in 2 ml tubes
- store at -20°C
|Reagent||for 1 L||500 mL||100 mL|
|2 M glucose||10 ml||5 ml||1 ml|
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.