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TOP10 chemically competent cells: Difference between revisions
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→Measurement of competence
Felix Moser (talk | contribs) |
Felix Moser (talk | contribs) |
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** Ampicillin and kanamycin appear to do fine with 1 hour growth | ** Ampicillin and kanamycin appear to do fine with 1 hour growth | ||
* Plate 20 μl on AMP plates using sterile 3.5 mm glass beads | * Plate 20 μl on AMP plates using sterile 3.5 mm glass beads | ||
* Transformation efficiency is 15 x colony count x 10<sup>5</sup> | ** Good cells should yield around 100 - 400 colonies | ||
** Transformation efficiency is (dilution factor=15) x colony count x 10<sup>5</sup> cfu/µgDNA | |||
**We expect that the transformation efficiency should be between 5x10<sup>8</sup> and 5x10<sup>9</sup> | **We expect that the transformation efficiency should be between 5x10<sup>8</sup> and 5x10<sup>9</sup> | ||
