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TOP10 chemically competent cells: Difference between revisions
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→Preparing competent cells
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** Flash freezing does not appear to be necessary | ** Flash freezing does not appear to be necessary | ||
* Test competence (see below) | * Test competence (see below) | ||
* Thawing and refreezing partially used cell aliquots dramatically reduces transformation efficiency by about 3x the first time, and about 6x total after several freeze/thaw cycles. | * Thawing and refreezing partially used cell aliquots dramatically reduces transformation efficiency by about 3x the first time, and about 6x total after several freeze/thaw cycles. | ||
