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DNA extraction - Salting Out: Difference between revisions
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→Reagents
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#Digestion Buffer (pH 8.0): 10mM NaCl, 10mM TRIS (pH 8.0), 10mM EDTA (pH 8.0), 0.5% SDS, | #Digestion Buffer (pH 8.0): 10mM NaCl, 10mM TRIS (pH 8.0), 10mM EDTA (pH 8.0), 0.5% SDS, | ||
#[[Proteinase K]] 20mg/mL | #[[Proteinase K]] 20mg/mL | ||
#[[Sodium | #[[Sodium acetate]] 3M (pH 5.2) | ||
#Ethanol 70% and 98% (chill prior to use) | #Ethanol 70% and 98% (chill prior to use) | ||
