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TOP10 chemically competent cells: Difference between revisions
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* Plate 20 μl on AMP plates using sterile 3.5 mm glass beads | * Plate 20 μl on AMP plates using sterile 3.5 mm glass beads | ||
* Transformation efficiency is 15 x colony count x 10<sup>5</sup> | * Transformation efficiency is 15 x colony count x 10<sup>5</sup> | ||
**We expect that the transformation efficiency should be between 5x10<sup>8</sup> and 5x10<sup>9</sup> | |||
==5x Ligation Adjustment Buffer== | ==5x Ligation Adjustment Buffer== | ||
