1,973
edits
TOP10 chemically competent cells: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Overview
| Line 2: | Line 2: | ||
This protocol is a variant of the Hanahan protocol <cite>Hanahan91</cite> using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the [[Media:pat6855494.pdf | Bloom05 patent]] as well. This protocol has been tested on TOP10, MachI and [[Talk:TOP10 chemically competent cells|BL21(DE3)]] cells. See [[Bacterial Transformation]] for a more general discussion of other techniques. The [[Media:pat6960464.pdf | Jesse '464 patent]] describes using this buffer for DH5α cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. | This protocol is a variant of the Hanahan protocol <cite>Hanahan91</cite> using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the [[Media:pat6855494.pdf | Bloom05 patent]] as well. This protocol has been tested on TOP10, MachI and [[Talk:TOP10 chemically competent cells|BL21(DE3)]] cells. See [[Bacterial Transformation]] for a more general discussion of other techniques. The [[Media:pat6960464.pdf | Jesse '464 patent]] describes using this buffer for DH5α cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. | ||
'''This is the chemical transformation protocol used by [[User:Tk|Tom Knight]] and the [http:// | '''This is the chemical transformation protocol used by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry of Standard Biological Parts]. | ||
==Materials== | ==Materials== | ||
